il 28b protein Search Results


92
Sino Biological recombinant mouse il 28b interferon lambda 3 sinobiological
Recombinant Mouse Il 28b Interferon Lambda 3 Sinobiological, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation recombinant mouse il-28b/ifn-lambda 3 protein, cf
Recombinant Mouse Il 28b/Ifn Lambda 3 Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems ifn l3
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R&D Systems 5259 il
5259 Il, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems recombinant human interleukin 28b
Immunofluorescence of IFI6- and IFN-λ2-expressing ZIKV-infected cells. (A) Huh7 cell lines expressing IFI6 or nontargeting sgRNAs were stained with an IFI6-specific antibody Scale bar = 20 μm. (B) Medium from Huh7 cell lines expressing IFN-λ2 or NT sgRNAs was used to treat Huh7 cells. After 18 h, the cells were harvested and analyzed by qPCR using Mx1 primers. <t>Recombinant</t> <t>IL-28b</t> (IFN-λ3, R-IL-28B) was used as a positive control (10 ng/μl). ***, P < 0.01; ****, P < 0.001, one-way ANOVA Dunnett’s multiple-comparison test. (C and D) Huh7 cell lines expressing IFI6, IFN-λ2, or nontargeting sgRNAs were infected with ZIKV (MOI, 10) (C) or DENV (MOI, 1) (D) for 2 h. After 24 h (ZIKV) or 48 h (DENV), the cells were fixed and stained with flavivirus protein E-specific antibody and an antibody recognizing dsRNA. DAPI was used for nucleus staining. Scale bar = 100 μm. The relative mean fluorescence intensity of each image compared to the control was quantified using Fiji and is presented below each image.
Recombinant Human Interleukin 28b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+28b+protein/Recombinant+Human+IL-28B%2FIFN-lambda+3+Protein/pmc06675891-494-12-21
Average 94 stars, based on 1 article reviews
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R&D Systems mouse recombinant ifn λ3
Immunofluorescence of IFI6- and IFN-λ2-expressing ZIKV-infected cells. (A) Huh7 cell lines expressing IFI6 or nontargeting sgRNAs were stained with an IFI6-specific antibody Scale bar = 20 μm. (B) Medium from Huh7 cell lines expressing IFN-λ2 or NT sgRNAs was used to treat Huh7 cells. After 18 h, the cells were harvested and analyzed by qPCR using Mx1 primers. <t>Recombinant</t> <t>IL-28b</t> (IFN-λ3, R-IL-28B) was used as a positive control (10 ng/μl). ***, P < 0.01; ****, P < 0.001, one-way ANOVA Dunnett’s multiple-comparison test. (C and D) Huh7 cell lines expressing IFI6, IFN-λ2, or nontargeting sgRNAs were infected with ZIKV (MOI, 10) (C) or DENV (MOI, 1) (D) for 2 h. After 24 h (ZIKV) or 48 h (DENV), the cells were fixed and stained with flavivirus protein E-specific antibody and an antibody recognizing dsRNA. DAPI was used for nucleus staining. Scale bar = 100 μm. The relative mean fluorescence intensity of each image compared to the control was quantified using Fiji and is presented below each image.
Mouse Recombinant Ifn λ3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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R&D Systems recombinant mouse ifn λ3
Immunofluorescence of IFI6- and IFN-λ2-expressing ZIKV-infected cells. (A) Huh7 cell lines expressing IFI6 or nontargeting sgRNAs were stained with an IFI6-specific antibody Scale bar = 20 μm. (B) Medium from Huh7 cell lines expressing IFN-λ2 or NT sgRNAs was used to treat Huh7 cells. After 18 h, the cells were harvested and analyzed by qPCR using Mx1 primers. <t>Recombinant</t> <t>IL-28b</t> (IFN-λ3, R-IL-28B) was used as a positive control (10 ng/μl). ***, P < 0.01; ****, P < 0.001, one-way ANOVA Dunnett’s multiple-comparison test. (C and D) Huh7 cell lines expressing IFI6, IFN-λ2, or nontargeting sgRNAs were infected with ZIKV (MOI, 10) (C) or DENV (MOI, 1) (D) for 2 h. After 24 h (ZIKV) or 48 h (DENV), the cells were fixed and stained with flavivirus protein E-specific antibody and an antibody recognizing dsRNA. DAPI was used for nucleus staining. Scale bar = 100 μm. The relative mean fluorescence intensity of each image compared to the control was quantified using Fiji and is presented below each image.
Recombinant Mouse Ifn λ3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+28b+protein/Recombinant+Mouse+IL-28B%2FIFN-lambda+3+Protein/pmc06249671-86-0-17
Average 93 stars, based on 1 article reviews
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R&D Systems ifn λ3
Immunofluorescence of IFI6- and IFN-λ2-expressing ZIKV-infected cells. (A) Huh7 cell lines expressing IFI6 or nontargeting sgRNAs were stained with an IFI6-specific antibody Scale bar = 20 μm. (B) Medium from Huh7 cell lines expressing IFN-λ2 or NT sgRNAs was used to treat Huh7 cells. After 18 h, the cells were harvested and analyzed by qPCR using Mx1 primers. <t>Recombinant</t> <t>IL-28b</t> (IFN-λ3, R-IL-28B) was used as a positive control (10 ng/μl). ***, P < 0.01; ****, P < 0.001, one-way ANOVA Dunnett’s multiple-comparison test. (C and D) Huh7 cell lines expressing IFI6, IFN-λ2, or nontargeting sgRNAs were infected with ZIKV (MOI, 10) (C) or DENV (MOI, 1) (D) for 2 h. After 24 h (ZIKV) or 48 h (DENV), the cells were fixed and stained with flavivirus protein E-specific antibody and an antibody recognizing dsRNA. DAPI was used for nucleus staining. Scale bar = 100 μm. The relative mean fluorescence intensity of each image compared to the control was quantified using Fiji and is presented below each image.
Ifn λ3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+28b+protein/Recombinant+Human+IL-28B%2FIFN-lambda+3+Protein%2C+CF/10__1002_slash_jlb__3a0120___001rrr-41-8-19
Average 90 stars, based on 1 article reviews
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R&D Systems ifn 3
Immunofluorescence of IFI6- and IFN-λ2-expressing ZIKV-infected cells. (A) Huh7 cell lines expressing IFI6 or nontargeting sgRNAs were stained with an IFI6-specific antibody Scale bar = 20 μm. (B) Medium from Huh7 cell lines expressing IFN-λ2 or NT sgRNAs was used to treat Huh7 cells. After 18 h, the cells were harvested and analyzed by qPCR using Mx1 primers. <t>Recombinant</t> <t>IL-28b</t> (IFN-λ3, R-IL-28B) was used as a positive control (10 ng/μl). ***, P < 0.01; ****, P < 0.001, one-way ANOVA Dunnett’s multiple-comparison test. (C and D) Huh7 cell lines expressing IFI6, IFN-λ2, or nontargeting sgRNAs were infected with ZIKV (MOI, 10) (C) or DENV (MOI, 1) (D) for 2 h. After 24 h (ZIKV) or 48 h (DENV), the cells were fixed and stained with flavivirus protein E-specific antibody and an antibody recognizing dsRNA. DAPI was used for nucleus staining. Scale bar = 100 μm. The relative mean fluorescence intensity of each image compared to the control was quantified using Fiji and is presented below each image.
Ifn 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+28b+protein/Recombinant+Human+IL-28B%2FIFN-lambda+3+Protein%2C+CF/10__1128_slash_jvi__02897___14-58-27-28
Average 90 stars, based on 1 article reviews
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91
Proteintech recombinant proteins animal
Immunofluorescence of IFI6- and IFN-λ2-expressing ZIKV-infected cells. (A) Huh7 cell lines expressing IFI6 or nontargeting sgRNAs were stained with an IFI6-specific antibody Scale bar = 20 μm. (B) Medium from Huh7 cell lines expressing IFN-λ2 or NT sgRNAs was used to treat Huh7 cells. After 18 h, the cells were harvested and analyzed by qPCR using Mx1 primers. <t>Recombinant</t> <t>IL-28b</t> (IFN-λ3, R-IL-28B) was used as a positive control (10 ng/μl). ***, P < 0.01; ****, P < 0.001, one-way ANOVA Dunnett’s multiple-comparison test. (C and D) Huh7 cell lines expressing IFI6, IFN-λ2, or nontargeting sgRNAs were infected with ZIKV (MOI, 10) (C) or DENV (MOI, 1) (D) for 2 h. After 24 h (ZIKV) or 48 h (DENV), the cells were fixed and stained with flavivirus protein E-specific antibody and an antibody recognizing dsRNA. DAPI was used for nucleus staining. Scale bar = 100 μm. The relative mean fluorescence intensity of each image compared to the control was quantified using Fiji and is presented below each image.
Recombinant Proteins Animal, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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90
Boster Bio ifn l2 il 28a
Immunofluorescence of IFI6- and IFN-λ2-expressing ZIKV-infected cells. (A) Huh7 cell lines expressing IFI6 or nontargeting sgRNAs were stained with an IFI6-specific antibody Scale bar = 20 μm. (B) Medium from Huh7 cell lines expressing IFN-λ2 or NT sgRNAs was used to treat Huh7 cells. After 18 h, the cells were harvested and analyzed by qPCR using Mx1 primers. <t>Recombinant</t> <t>IL-28b</t> (IFN-λ3, R-IL-28B) was used as a positive control (10 ng/μl). ***, P < 0.01; ****, P < 0.001, one-way ANOVA Dunnett’s multiple-comparison test. (C and D) Huh7 cell lines expressing IFI6, IFN-λ2, or nontargeting sgRNAs were infected with ZIKV (MOI, 10) (C) or DENV (MOI, 1) (D) for 2 h. After 24 h (ZIKV) or 48 h (DENV), the cells were fixed and stained with flavivirus protein E-specific antibody and an antibody recognizing dsRNA. DAPI was used for nucleus staining. Scale bar = 100 μm. The relative mean fluorescence intensity of each image compared to the control was quantified using Fiji and is presented below each image.
Ifn L2 Il 28a, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+28b+protein/Human+recombinant+IL-28B+(Interleukin-28B)+protein%2C+AF/10__1165_slash_rcmb__2020___0401oc-58-21-23
Average 90 stars, based on 1 article reviews
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Kingfisher Biotech common vampire bat ifn lambda 3 (il-28b) recombinant protein
Immunofluorescence of IFI6- and IFN-λ2-expressing ZIKV-infected cells. (A) Huh7 cell lines expressing IFI6 or nontargeting sgRNAs were stained with an IFI6-specific antibody Scale bar = 20 μm. (B) Medium from Huh7 cell lines expressing IFN-λ2 or NT sgRNAs was used to treat Huh7 cells. After 18 h, the cells were harvested and analyzed by qPCR using Mx1 primers. <t>Recombinant</t> <t>IL-28b</t> (IFN-λ3, R-IL-28B) was used as a positive control (10 ng/μl). ***, P < 0.01; ****, P < 0.001, one-way ANOVA Dunnett’s multiple-comparison test. (C and D) Huh7 cell lines expressing IFI6, IFN-λ2, or nontargeting sgRNAs were infected with ZIKV (MOI, 10) (C) or DENV (MOI, 1) (D) for 2 h. After 24 h (ZIKV) or 48 h (DENV), the cells were fixed and stained with flavivirus protein E-specific antibody and an antibody recognizing dsRNA. DAPI was used for nucleus staining. Scale bar = 100 μm. The relative mean fluorescence intensity of each image compared to the control was quantified using Fiji and is presented below each image.
Common Vampire Bat Ifn Lambda 3 (Il 28b) Recombinant Protein, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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Image Search Results


Immunofluorescence of IFI6- and IFN-λ2-expressing ZIKV-infected cells. (A) Huh7 cell lines expressing IFI6 or nontargeting sgRNAs were stained with an IFI6-specific antibody Scale bar = 20 μm. (B) Medium from Huh7 cell lines expressing IFN-λ2 or NT sgRNAs was used to treat Huh7 cells. After 18 h, the cells were harvested and analyzed by qPCR using Mx1 primers. Recombinant IL-28b (IFN-λ3, R-IL-28B) was used as a positive control (10 ng/μl). ***, P < 0.01; ****, P < 0.001, one-way ANOVA Dunnett’s multiple-comparison test. (C and D) Huh7 cell lines expressing IFI6, IFN-λ2, or nontargeting sgRNAs were infected with ZIKV (MOI, 10) (C) or DENV (MOI, 1) (D) for 2 h. After 24 h (ZIKV) or 48 h (DENV), the cells were fixed and stained with flavivirus protein E-specific antibody and an antibody recognizing dsRNA. DAPI was used for nucleus staining. Scale bar = 100 μm. The relative mean fluorescence intensity of each image compared to the control was quantified using Fiji and is presented below each image.

Journal: Journal of Virology

Article Title: A CRISPR Activation Screen Identifies Genes That Protect against Zika Virus Infection

doi: 10.1128/JVI.00211-19

Figure Lengend Snippet: Immunofluorescence of IFI6- and IFN-λ2-expressing ZIKV-infected cells. (A) Huh7 cell lines expressing IFI6 or nontargeting sgRNAs were stained with an IFI6-specific antibody Scale bar = 20 μm. (B) Medium from Huh7 cell lines expressing IFN-λ2 or NT sgRNAs was used to treat Huh7 cells. After 18 h, the cells were harvested and analyzed by qPCR using Mx1 primers. Recombinant IL-28b (IFN-λ3, R-IL-28B) was used as a positive control (10 ng/μl). ***, P < 0.01; ****, P < 0.001, one-way ANOVA Dunnett’s multiple-comparison test. (C and D) Huh7 cell lines expressing IFI6, IFN-λ2, or nontargeting sgRNAs were infected with ZIKV (MOI, 10) (C) or DENV (MOI, 1) (D) for 2 h. After 24 h (ZIKV) or 48 h (DENV), the cells were fixed and stained with flavivirus protein E-specific antibody and an antibody recognizing dsRNA. DAPI was used for nucleus staining. Scale bar = 100 μm. The relative mean fluorescence intensity of each image compared to the control was quantified using Fiji and is presented below each image.

Article Snippet: Interferon alpha B2 was purchased from PBL (NJ; catalog no. 11115-1), and recombinant human interleukin 28B (IL-28B)/IFN-λ 3 protein was from R&D Systems (catalog no. 5259-IL). . sgRNA library amplification and lentivirus production.

Techniques: Immunofluorescence, Expressing, Infection, Staining, Recombinant, Positive Control, Comparison, Fluorescence, Control